Human IL-18 Protein (IL1-HE018)
Catalogue Number: IL1-HE018-KAT
IL1-HE018 Image
IL1-HE018 Image
IL1-HE018 Image
IL1-HE018 Image
IL1-HE018 Image
IL1-HE018 Image
Human IL-18 R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-18, No Tag with an affinity constant of 2.38 nM as determined in SPR assay (Biacore T200).
IL1-HE018 Image
The purity of Human IL-18 is greater than 95% as determined by SEC-HPLC.
IL1-HE018 Image
Human IL-18 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
IL1-HE018 Image
Immobilized Human IL-18 at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-18BP, hFc Tag with the EC50 of 62.0ng/ml determined by ELISA (QC Test).
IL1-HE018 Image
Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50-for this effect is 1-5 ng/mL (QC Test).
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 18.2 kDa same as Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Interleukins |
| Alias: | IL-18; IL18; IGIF; IL-1F4; IL-1 gamma; IL1F4; IL-1g; Interleukin 1; IL-1γ; IL-1 γ |
| Host Cell: | E.coli |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naive T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation.
Additional Text
Gene Name
IL18
Gene ID
3606
Uniprot ID
Q14116
Protein Tag
Untagged
Short Description
Recombinant Human IL-18 Protein is expressed from E.coli without tag.It contains Tyr37-Asp193.
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biological Activity
Immobilized Human IL-18 at 5Oeg/ml (100Oel/Well) on the plate. Dose response curve for Human IL-18BP, hFc Tag with the EC50 of 18.2ng/ml determined by ELISA. The affinity constant of 2.38 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Endotoxin
Less than 0.1 EU per µg by the LAL method.
IL1-HE018 Datasheet
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