Human IL-27 Protein (IL2-HM127)
Catalogue Number: IL2-HM127-KAT
IL2-HM127 Image
IL2-HM127 Image
IL2-HM127 Image
IL2-HM127 Image
Human IL-27 RA, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-27, His Tag with an affinity constant of 3.58 nM as determined in SPR assay (Biacore T200).
IL2-HM127 Image
Human IL-27 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
IL2-HM127 Image
Immobilized Human IL-27 RA, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 22.1ng/ml determined by ELISA (QC Test).
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 50.6 kDa. Due to glycosylation, the protein migrates to 54-64 kDa based on Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Interleukins |
| Alias: | Interleukin-27; p28; IL30; IL-27; IL-27A; IL27; p28 |
| Host Cell: | CHO Cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: Interleukin (IL)-27, a member of the IL-6/IL-12 family, has an important role in modulating inflammation in partnership with innate and adaptive immune cells.
Additional Text
Protein Tag
HIS Tag
Gene ID
10148; 246778
Uniprot ID
Q14213, Q8NEV9
Gene Name
EBI3, IL27
Short Description
Recombinant Human IL-27 Protein is expressed from CHO with His tag at the C-Terminus.It contains Arg21-Lys229(IL-27B)&Phe29-Pro243(IL-27A).
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biological Activity
Immobilized Human IL-27 RA, hFc Tag at 5Oeg/ml (100Oel/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 16.8ng/ml determined by ELISA. The affinity constant of 3.58 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Endotoxin
Less than 1 EU per µg by the LAL method.
IL2-HM127 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations