Human IL-15 Protein (IL5-HE015)
Catalogue Number: IL5-HE015-KAT
IL5-HE015 Image
IL5-HE015 Image
IL5-HE015 Image
IL5-HE015 Image
IL5-HE015 Image
IL5-HE015 Image
The purity of Human IL-15 is greater than 95% as determined by SEC-HPLC.
IL5-HE015 Image
Human IL-2 R beta, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-15, No Tag with an affinity constant of 5.9 nM as determined in SPR assay (Biacore T200).
IL5-HE015 Image
Human IL-15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
IL5-HE015 Image
Immobilized Human IL-15, No Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human IL-15RA, His Tag with the EC50 of 15.4 ng/ml determined by ELISA.
IL5-HE015 Image
Measured in a cell proliferation assay using CTLL-2 cells. The ED50-for this effect is 0.1 - 0.3 ng/mL. The specific activity of recombinant human IL-15 is > 1 x 10^7-units/mg, which is calibrated against the human IL-15 reference standard (NIBSC code: 95/554) (QC Test).
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 12.8 kDa same as Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Interleukins |
| Alias: | L15; IL-15; IL-15MGC9721; Interleukin-15 |
| Host Cell: | E.coli |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases. IL-15 is a cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA.
Additional Text
Gene ID
3600
Uniprot ID
P40933
Gene Name
IL15
Protein Tag
Untagged
Short Description
Recombinant Human IL-15 Protein is expressed from E.coli without tag.It contains Asn49-Ser162.
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biological Activity
Immobilized Human IL-15 at 1Oeg/ml (100Oel/Well) on the plate. Dose response curve for Human IL-15RA, His Tag with the EC50 of 12.1ng/ml determined by ELISA. The affinity constant of 0.056 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Endotoxin
Less than 0.05 EU per µg by the LAL method.
IL5-HE015 Datasheet
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