Human IL-6 Protein (IL6-HE001)
Catalogue Number: IL6-HE001-KAT
IL6-HE001 Image
IL6-HE001 Image
IL6-HE001 Image
IL6-HE001 Image
IL6-HE001 Image
IL6-HE001 Image
IL6-HE001 Image
Human IL-6 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-6, No Tag with an affinity constant of 0.22 nM as determined in SPR assay (Biacore T200).
IL6-HE001 Image
The purity of Human IL-6 is greater than 90% as determined by SEC-HPLC.
IL6-HE001 Image
Human IL-6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
IL6-HE001 Image
Immobilized Human IL-6 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 6.0ng/ml determined by ELISA.
IL6-HE001 Image
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.1 - 0.8 ng/mL.
IL6-HE001 Image
Loaded Human IL-6 R alpha, hFc Tag on ProA-Biosensor can bind Human IL-6, No Tag with an affinity constant of 16.50 nM as determined in BLI assay (Gator® Prime).
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted 20.8 kDa same as Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Interleukins |
| Alias: | IFN-beta-2; IL6; IL-6; BSF-2; CDF; MGI-2A; Interleukin-6; HSF; IFNB2; HGF;IFN-β-2 |
| Host Cell: | E.coli |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression.Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein.
Additional Text
Gene Name
IL6
Gene ID
3569
Uniprot ID
P05231
Protein Tag
Untagged
Short Description
Recombinant Human IL-6 is expressed from E.coli without tag.It contains Val30-Met212.
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biological Activity
Immobilized Human IL-6 at 0.5Oeg/ml (100Oel/Well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 32.6ng/ml determined by ELISA. The affinity constant of 0.22 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Endotoxin
Less than 0.05 EU per µg by the LAL method.
IL6-HE001 Datasheet
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