Human IL-7 Protein (IL7-HE001)
Catalogue Number: IL7-HE001-KAT
IL7-HE001 Image
IL7-HE001 Image
IL7-HE001 Image
IL7-HE001 Image
IL7-HE001 Image
IL7-HE001 Image
Human IL-7 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-7, No Tag with an affinity constant of 7.15 nM as determined in SPR assay (Biacore T200).
IL7-HE001 Image
Human IL-7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
IL7-HE001 Image
The purity of Human IL-7 is greater than 95% as determined by SEC-HPLC.
IL7-HE001 Image
Immobilized Human IL-7 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 16.2ng/ml determined by ELISA (QC tested).
IL7-HE001 Image
Measured in a cell proliferation assay using murine 2E8 cells. The ED50-for this effect is-0.1 - 0.5 ng/mL (QC tested).
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 17.5 kDa same as Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Interleukins |
| Alias: | IL7; Interleukin-7; IL-7; Lymphopoietin-1; PBGF |
| Host Cell: | E.coli |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: Interleukin-7 (IL7) plays a nonredundant role in T cell survival and homeostasis, which is illustrated in the severe T cell lymphopenia of IL7-deficient mice, or demonstrated in animals or humans that lack expression of either the IL7Rα or γ c chain, the two subunits that constitute the functional IL7 receptor.
Additional Text
Gene Name
IL7
Gene ID
3574
Uniprot ID
P13232
Protein Tag
Untagged
Short Description
Recombinant Human IL-7 Protein is expressed from E.coli without tag.It contains Asp26-His177.
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biological Activity
Immobilized Human IL-7 at 0.5Oeg/ml (100Oel/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 0.21Oeg/ml determined by ELISA. The affinity constant of 22.66 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Endotoxin
Less than 0.01 EU per µg by the LAL method.
IL7-HE001 Datasheet
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