Catalogue Number: K1032-APE
Manufacturer: | Apexbio |
Type: | DNA Polymerases |
Shipping Condition: | Dry Ice |
Unit(s): | 100 U, 1000 U, 500 U |
Description: hyPerFUsion™ high-fidelity DNA polymerases are composed of a DNA-binding domain fused to a Pyrococcus-like proofreading polymerase. Because of this, hyPerFUsion™ DNA polymerases produce PCR products with very high speed and accuracy. Also, hyPerFUsion™ DNA polymerases are tolerant of various inhibitors, allowing for robust amplification with minimal optimization. hyPerFUsion™ DNA Polymerase is an ideal choice for cloning and can be used for long or difficult (GC-rich) amplicons. hyPerFUsion™ high-fidelity DNA polymerases have become the choice for even demanding applications like massively parallel high-throughput sequencing of whole genomes.High-Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. hyPerFUsion™ DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate >50 fold lower than that of Taq DNA Polymerase and 6 fold lower than that of Pyrococcus furiosus DNA Polymerase (1), hyPerFUsion™ is one of the most accurate thermostable polymerases available. hyPerFUsion™ DNA Polymerase possesses 5' to 3' polymerase activity, 3' to 5' exonuclease activity and will generate blunt-ended products.hyPerFUsion™ DNA Polymerase is supplied with standard 5X hyPerFUsion™ Buffer, which can be used for complex or GC-rich templates. Reactions can also be optimized using the provided DMSO.
Store at -20°C.