HyperScribe™ T7 High Yield Cy5 RNA Labeling Kit

Catalogue Number: K1062-APE

Type:Nucleic Acid Labeling Kits
Shipping Condition:Dry Ice
Unit(s): 25 reactions


Description: HyperScribe™ T7 High Yield Cy5 RNA Labeling Kit is designed to generate randomly Cy5-modified RNA probes by in vitro transcription. Such probes are ideally suited for in situ hybridization and Northern blot hybridization experiments. The principle of labeling is similar to the basic labeling principle of a mixture of Cy5 RNA labels. Cy5-UTP is efficiently incorporated into RNA using the optimized reaction buffer and T7 RNA polymerase mixture in place of its natural counterpart UTP. An appropriate Cy5-UTP substitution typically achieves an optimal balance between reaction and labeling efficiency. However, the individual optimization of the Cy5-UTP/UTP ratio can be easily achieved using a single nucleotide format. The resulting Cy5 -modified RNA probe can then be detected by fluorescence spectroscopy.

Additional Text

Short Description

RNA probe for Cy5 modification obtained by in vitro transcription

Storage Note

Store all the kit components at -20°C.


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