Reverse Transcriptase is a new enzyme obtained through genetic engineering based on M-MLV Reverse Transcriptase. In comparison, Reverse Transcriptase reduces RNase H activity and increases thermal stability. Reverse Transcriptase can withstand higher reaction temperatures and is suitable for reverse transcription of RNA templates with complex secondary structures. In addition, Reverse Transcriptase enhances the affinity to the template, suitable for the reverse transcription of a small number of templates and low copy genes, and can generate cDNA up to 12.3 kb.
Reverse Transcriptase is generally used to achieve reverse transcription of RNA into cDNA for subsequent qPCR experiments or other experiments.
Reverse Transcriptase (200 U/μL)
5x First-Strand Buffer
Good thermal stability and high reverse transcription efficiency
Suitable for RNA templates with secondary structure
The template has a strong affinity for more full-length cDNA
Highly sensitive to detect total RNA down to 10 pg
Thermally stable reverse transcriptase used to synthesize complementary DNA (cDNA) from an RNA template.