Catalogue Number: K1072-APE
|Type:||cDNA Synthesis Kits|
|Shipping Condition:||Blue Ice|
|Unit(s):||20 reactions, 50 reactions, 100 reactions|
Description: The First-Strand cDNA Synthesis Kit is based on Reverse Transcriptase (Cat. No. K1071) to synthesize first-strand cDNA from Total RNA or Poly(A)+ RNA. Reverse Transcriptase is a new enzyme obtained through genetic engineering based on M-MLV (RNase H-) Reverse Transcriptase. In comparison, Reverse Transcriptase reduces RNase H activity and increases thermal stability. Reverse Transcriptase can withstand higher reaction temperatures and is suitable for reverse transcription of RNA templates with complex secondary structures. In addition, Reverse Transcriptase enhances the affinity to the template, suitable for the reverse transcription of a small number of templates and low copy genes, and can generate cDNA up to 12.3 kb.The First-Strand cDNA Synthesis Kit contains all the components needed to synthesize the first-strand cDNA and provides two cDNA synthesis primers, Random Primers and Oligo (dT)23VN. You can choose Random Primers, Oligo (dT)23VN or gene-specific primers as reverse transcription primers according to the needs of the experiment. The synthesized first-strand cDNA products can be used in subsequent experiments such as PCR amplification and qPCR reaction.
Synthesize first-strand cDNA from purified poly(A)+ or total RNA
Store the components at -20°C.