Luciferase Reporter Assay Kit
Catalogue Number: K2181-APE
| Manufacturer: | Apexbio |
| Type: | Luciferase Reporter Detection Kits |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 assays |
Description
Description: Firefly luciferase catalyzes the highly efficient oxidative carboxylation of luciferin. The light emission of this biolumninescence reaction can be detected by a luminometer. Firefly luciferase is a sensitive reporter for gene regulation and function study. The Luciferase Reporter Assay Kit offers a fast and easy way of sensitive way of detecting lucifeasue activity in transfected eukaryotic cells. For measurement of expressed luciferase in vitro, luciferase is first extracted from transfected cells through cell lysis. Then CoA, ATP, Mg²+ and buffer are added to the lysate. Then the injection of luciferin triggers the luminescent reaction which the emitted light can be detected by luminometer. This Luciferase Reporter Assay includes CoA, ATP and Mg²+ in an optimized buffer solution, to ensure highest sensitivity, consistent light output , plus convenience and consistency for multiple samples.
Additional Text
Storage Note
Store at -20°C.
K2181 Datasheet
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