Universal Loading Control Antibody Cocktail

Catalogue Number: KTD101-EN-ABK

Manufacturer:Abbkine
Shelf Life:12 months
Type:Primary Antibody
Shipping Condition:Blue Ice
Unit(s): 1 kit

Description

Description: The Universal Internal Reference Antibody Cocktail is a set containing the antibodies β-actin, GAPDH and β-tubulin commonly used in immunoassay (WB, IHC-P, IF) as well as the antibody diluent. There are many kinds of internal reference antibodies. Therefore, the following three principles should be followed in choosing an appropriate internal reference antibody: 1.Considering the sample species, for mammalian tissues and cells, the commonly used reference proteins are β-actin, Tubulin, GAPDH, Lamin B1, PCNA, Na+/K+-ATPase, etc.For Plant samples, Plant Actin and RuBisCo are commonly used.Consider the molecular weight of the target protein. 2. The selected reference protein should have a different molecular weight from the target protein (>5 kDa) to distinguish the target protein bands. For high molecular weight proteins (over 100 kDa), we recommend that our clients use Vinculin as an internal reference; 2. Considering the expression and localization of target protein, select the corresponding internal reference, such as whole cell, nucleus, mitochondria, cell membrane and so on.

Additional Text

Short Description

Loading Control