Universal IP/Co-IP Toolkit (Magnetic Beads)

Catalogue Number: KTD104-EN-ABK

Manufacturer:Abbkine
Type:Protein Assays & Kits
Shipping Condition:Blue Ice
Unit(s): 20 T
Range:
Sensitivity:
Sample type:
Sample size:

Description

Description:

Applications notesBased on the pain points of the traditional IP/Co-IP experiment, Abbkine developed a Universal IP/Co-IP Toolkit (Magnetic Beads); After designed and researched, the toolkit contains: optimized natural and denatured lysate, protein A/G magnetic beads, IP negative control, IPkineTM second antibody, it can meet the IP/Co-IP needs of most users

Product Properties

Kit components• Non-Denaturing Lysis Buffer
• Denaturing Lysis Buffer
• 20×Wash Buffer
• Protein A/G Magnetic Beads
• Elution Buffer
• Neutralization Buffer
• 100×Proteinase Inhibitor Cocktail
• Mouse IgG (1 mg/mL)
• Rabbit IgG (1mg/mL)
• IPKine™ HRP, Goat Anti-Mouse IgG LCS
• IPKine™ HRP, Mouse Anti-Rabbit IgG LCS
Features & Benefits• Efficient: Efficient antibody binding capacity and low protein non-specific adsorption rate, saving antibody consumption.
• Convenient and universal: Contain all the necessary buffers needed for IP/Co-IP and WB experiments, it can meet the requirements of sample IP/Co-IP or WB at the same time.
• Reliable and stable: It contains ready-to-use IP negative control, which can eliminate the non-specific binding of IgG itself to the target protein or other specific biomolecules to ensure the specificity of IP antibodies, and contains unique IPkine™ secondary antibodies to perfectly eliminate heavy chain interference.
Storage instructionsRefer to list of materials supplied for storage conditions of individual components.
ShippingBlue ice
PrecautionsThe product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

BackgroundImmunoprecipitation (Immunoprecipitation, IP) is a small affinity purification method using specific antibodies fixed on solid-phase supports such as magnetic beads or agarose. As an important part of many proteomics-related studies, IP can be used to detect the existence of proteins, relative abundance, up-and-down expression of proteins, stability and interaction of proteins and so on.