Protein Quantification Kit (Bradford Assay)
Catalogue Number: KTD3002-ABK
| Manufacturer: | Abbkine |
| Type: | Protein Detection |
| Alias: | Coomassie |
| Shipping Condition: | Blue Ice |
| Unit(s): | 2000 tests, 500 T, 5000 T |
Description
Description: The Bradford assay, a colorimetric protein assay, is based on an absorbance shift of the dye Coomassie Brilliant Blue G-250. The Coomassie Brilliant Blue G-250 dye exists in three forms: anionic (blue), neutral (green), and cationic (red). Under acidic conditions, the red form of the dye is converted into its blue form, binding to the protein being assayed. If there's no protein to bind, then the solution will remain brown. The cationic (unbound) form is green / red and has an absorption spectrum maximum historically held to be at 465 nm. The anionic bound form of the dye which is held together by hydrophobic and ionic interactions, has an absorption spectrum maximum historically held to be at 595 nm. The increase of absorbance at 595 nm is proportional to the amount of bound dye, and thus to the amount (concentration) of protein present in the sample.
Additional Text
Detection Method
Colorimetric
Short Description
Abbkine Protein Quantification Kit (Bradford Assay) provides a simple and rapid procedure for determining the concentration of protein in samples.
KTD3002 Datasheet
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