ExKine™ Nuclear Protein Extraction Kit
Catalogue Number: KTP3002-ABK
KTP3002 Image
KTP3002 Image
Fig. Western blot of specific proteins fraction using Abbkine ExKine™ Nuclear Protein Extraction Kit. Sample: 293T cells lysate. C: cytoplasmic extraction was analyzed using Histone H3 antibody (A01070). N: nuclear extraction was analyzed using Histone H3 antibody (A01070).
| Manufacturer: | Abbkine |
| Type: | Protein Extraction Kit |
| Alias: | Nuclear|Nuclear Protein |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 T, 50 T |
| Application: | IP, WB |
Description
Description: The preparation of an extract from nuclei is often the first step in studying nuclear proteins and their interactions. The resulting preparation can be used directly in the Electrophoresis Mobility Shift Assay (EMSA), footprinting analysis, transcription assays, or as a starting point for the purification of regulatory proteins.
Additional Text
Short Description
Abbkine ExKine™ Nuclear Protein Extraction Kit enable stepwise separation and preparation of crude cytoplasmic and nuclear extracts from mammalian cultured cells or tissue.
Note
Perform all steps at 2-8 °C. Use precooled buffers and equipment. Ensure all the solutions are defrosted and homogeneous.
KTP3002 Datasheet
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