Human LILRB4/CD85k/ILT3 Protein (LIL-HM2B4)
Catalogue Number: LIL-HM2B4-KAT
LIL-HM2B4 Image
LIL-HM2B4 Image
LIL-HM2B4 Image
LIL-HM2B4 Image
LIL-HM2B4 Image
Human LILRB4, hFc Tag captured on CM5 Chip via Protein A can bind Human APOE 4, His Tag with an affinity constant of 74.20 nM as determined in SPR assay (Biacore T200).
LIL-HM2B4 Image
The purity of Human LILRB4 is greater than 95% as determined by SEC-HPLC.
LIL-HM2B4 Image
Human LILRB4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
LIL-HM2B4 Image
Immobilized Human LILRB4, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-LILRB4 Antibody, hFc Tag with the EC50 of 27.3ng/ml determined by ELISA (QC Test).
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 52.6 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Other Recombinant Protein |
| Alias: | HM18; ILT3; ILT-3; LILRB4; LIR5; CD85K |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: LILRB4,also known as CD85k and LIR-5, ILT3, is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation. Mature human ILT3 consists of a 238 amino acid (aa) extracellular domain with two Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM).LILRB4 is receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles.
Additional Text
Protein Tag
hFC Tag
Short Description
Recombinant Human LILRB4/CD85k/ILT3 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln22-Arg256.
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biological Activity
Immobilized Human LILRB4, hFc Tag at 0.2Oeg/ml (100Oel/well) on the plate. Dose response curve for Biotinylated Anti-LILRB4 Antibody, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA. The affinity constant of 74.20 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Endotoxin
Less than 1 EU per µg by the LAL method.
LIL-HM2B4 Datasheet
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