Actiflash

Catalogue Number: LJU-CIN-5-IDY

Manufacturer:Idylle Labs
Type:Biological dyes
Shipping Condition:RT
Unit(s): 5 mg
Application: InVivoTrk

Description

Description: Actiflash is a photo-inducible tamoxifen analogue. Upon UV-light irradiation, the compound activates your chosen ERT2 protein fusion. It can be used to control transcription (using Gal4-UAS) or induce recombination (using Cre-lox) in space and/or time for in-vivo cell tracking experiments.

Wide applicative scope

Technology capitalizing on the versatile use of Tamoxifen-OH for controlling functions of multiple types of proteins.

Photochemical stability
Caged Cyclofen-OH liberates Cyclofen-OH, a highly photostable compound in contrast to Tamoxifen-OH encountering photodegradation under illumination.

Favorable wavelength ranges for uncaging
Uncaging requires either UV-A light or a strong IR laser. Visible light is inactive, which facilitates the experiments with biological samples.

Simple conditioning
Caged Cyclofen-OH is cell-permeant and can be added either in the external medium or directly injected for conditioning.

Excellent chemical stability
Caged Cyclofen-OH does not generate any basal activation of protein function and it benefits from an excellent temporal resolution upon uncaging.

Applications
Actiflash is a great R&D tool to convert your inducible ERT model into a photo-inducible one. It is very helpful if you want to control transcription (using Gal4-UAS) or induce recombination (using Cre-lox) in space and/or time for in-vivo cell tracking experiments and more.
Actiflash has been successfully used with various cell lines as well as zebrafish embryos. Actiflash will work best on zebrafish embryos from 12 to 48hpf. 

Technical Information
Lifetime: upon receipt, Actiflash is stable for years at 2-8°C. Once solubilized in a DMSO solution (typically at 10 mM), Actiflash can be stored at -20°C for several months.  Always wrap the vials containing the aliquoted Actiflash with aluminium foil to protect it from light. 

Additional Text

Short Description

Tamoxifen-like photoactivable inducer

LJU-CIN-5 MSDS

LJU-CIN-5 Reference

Price
£570.00


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