PathPlus™ PRKCZ / PKC-Zeta Antibody (N-Terminus)

Catalogue Number: LS-A8392-LSP

Manufacturer:LifeSpan BioSciences Inc.
Shelf Life:12 months
Preservative:0.1% Sodium azide
Type:Polyclonal Primary Antibody - Unconjugated
Alias:PRKCZ, PKC-ZETA, PKC2, Pkcz, PKCzeta, Protein kinase c zeta, Protein kinase C zeta type, NPKC-zeta, PKC Zeta, Protein kinase C, zeta, Protein kinase Czeta
Shipping Condition:RT
Unit(s): 50 ug
Host name: Rabbit
Clone:
Isotype:
Immunogen: Synthetic 19 amino acid peptide near N-Terminus of human PRKCZ / PKC-Zeta. Percent identity with other species by BLAST analysis: Human, Gorilla (100%); Monkey (95%); Marmoset, Mouse, Rat, Rabbit (89%).
Application: ELISA, IHC-P, IHC

Additional Text

Concentration

1 mg/ml

Gene ID

5590

Gene Name

PRKCZ

Antigen Type

Synthetic Peptide

Purification

Affinity Purified

Protein: Target Region

N-terminus

Short Description

PKC-Zeta antibody LS-A8392 is an unconjugated rabbit polyclonal antibody to human PKC-Zeta (PRKCZ) (N-Terminus). Validated for ELISA and IHC.

Antibody Clonality

Polyclonal

Storage Note

Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Application Notes

Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 2.5-3 ug/ml.