IHC-plus™ NF-L / NEFL Antibody
Catalogue Number: LS-B10376-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Preservative: | Sodium azide |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | NEFL, 68 kDa neurofilament protein, CMT1F, CMT2E, NF68, NF-L, NFL, Neurofilament subunit NF-L, PPP1R110, Neurofilament light polypeptide, CMTDIG |
| Shipping Condition: | RT |
| Unit(s): | 50 ul |
| Host name: | Chicken |
| Clone: | |
| Isotype: | IgY |
| Immunogen: | To raise this antibody bovine intermediate filaments were prepared from spinal cords by the method of Delacourte et al. and the cytoskeletal material was dissolved in 6M urea. Individual neurofilament subunits were purified by ion exchange chromatography on DEAE cellulose followed by preparative gel electrophoresis. To ensure greater specificity for NF-L, animals were boosted with recombinant mouse NF-L purified from bacteria. |
| Application: | ICC, IF, IHC-P, WB, IHC |
Additional Text
Gene Name
NEFL
Gene ID
4747
Antigen Type
Purified protein
Purification
Purified
Short Description
NEFL antibody LS-B10376 is an unconjugated chicken polyclonal antibody to human NEFL (NF-L). Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Storage Note
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Antibody Clonality
Polyclonal
Application Notes
The IgY solution can be used at dilutions of at least 1:5000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:10000 or lower.
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