IHC-plus™ MAPK6 / ERK3 Antibody (aa683-694)
Catalogue Number: LS-B10395-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Preservative: | 0.02% Sodium azide |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | MAPK6, ERK-3, MAP kinase 6, MAPK 6, p97-MAPK, PRKM6, MAP kinase isoform p97, ERK3, HsT17250, p97MAPK |
| Shipping Condition: | RT |
| Unit(s): | 50 ug |
| Host name: | Goat |
| Clone: | |
| Isotype: | |
| Immunogen: | Peptide with sequence C-HSPVGSPLKSIQ, from the internal region (near C-Terminus) of the protein sequence according to NP_002739.1. |
| Application: | IHC-P, IP, WB, IHC, pELISA |
Additional Text
Gene ID
5597
Gene Name
MAPK6
Concentration
0.5 mg/ml
Purification
Ammonium Sulfate Precipitated; Affinity Purified
Short Description
ERK3 antibody LS-B10395 is an unconjugated goat polyclonal antibody to ERK3 (MAPK6) (aa683-694) from human. It is reactive with human, mouse, bovine and other species. Validated for IHC, IP, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Antibody Clonality
Polyclonal
Storage Note
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Application Notes
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: In transfected HEK293 transiently expressing Mouse ERK3 / Mapk6 fused to GFP the band with expected size is intensified when co-expressed with MK5 as the stabilizer for ERK3. No bands are observed in transfected HEK293 with empty vector and the same band is observed using anti-GFP antibody. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration, 0.5-1 ug/ml. Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced with MK5/ERK3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5 ug EB11926. Western blots of the IP were labeled with rabbit anti-ERK3 serum (CST) or with mouse anti-MK5 (SC). An approx 100kD of ERK3 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression construct as described in as described in PMID: 22508986. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.
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