IHC-plus™ MAPKAPK2 / MAPKAP Kinase 2 Antibody (aa339-351)

Catalogue Number: LS-B10396-LSP

Manufacturer:	LifeSpan BioSciences Inc.
Preservative:	0.02% Sodium azide
Type:	Polyclonal Primary Antibody - Unconjugated
Alias:	MAPKAPK2, MK-2, MAP KAP Kinase 2, MAPKAP kinase 2, MK2, MAPKAP-K2, MAPKAPK-2
Shipping Condition:	RT
Unit(s):	50 ug
Host name:	Goat
Clone:
Isotype:
Immunogen:	Peptide with sequence C-SRVLKEDKERWED, from the internal region (near C-Terminus) of the protein sequence according to NP_004750.1NP_116584.2.
Application:	IHC-P, IP, WB, IHC, pELISA

Overview

Additional Text

Gene ID

9261

Gene Name

MAPKAPK2

Concentration

0.5 mg/ml

Purification

Ammonium Sulfate Precipitated; Affinity Purified

Short Description

MAPKAP Kinase 2 antibody LS-B10396 is an unconjugated goat polyclonal antibody to MAPKAP Kinase 2 (MAPKAPK2) (aa339-351) from human. It is reactive with human, mouse, bovine and other species. Validated for IHC, IP, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.

Antibody Clonality

Polyclonal

Storage Note

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Application Notes

Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 60kD band observed in Mouse Embryonic Fibroblasts (MEF) lysates, not present in the knockout MEFs and reappearing as a 45kD band upon reintroduction of MK2 gene expression through viral transduction (calculated MW of 44.0kD according to Mouse NP_032577.1). The observed molecular weights correspond to earlier findings in literature with different antibodies (Menon et al, Cell Motil Cytoskeleton. 2009 Dec;66(12):1041-7. PMID: 19743408). There is no cross-reactivity to MK5. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration: 0.5-2 ug/ml. Immunoprecipitation: MK2/MK3 double knockout MEFs retrovirally transduced with MK2 or empty vector were lysed from confluent plates and used for IP with 1.5 ug EB11929 or EB11930. Western blots of the IP were labeled with rabbit anti-MK2 (CST). An approx 45kD of MK2 is only precipitated from lysates of those KO MEFs that have been rescued by the MK2 expression construct as described in PMID: 19743408. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.

Price

£391.00

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