IHC-plus™ HIF1A / HIF1 Alpha Antibody (aa329-530, clone ESEE122)
Catalogue Number: LS-B109-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Preservative: | 0.05% Sodium azide |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | HIF1A, ARNT-interacting protein, ARNT interacting protein, BHLHe78, HIF1 Alpha, Hypoxia-inducible factor1alpha, HIF-1A, HIF1, MOP1, Member of PAS superfamily 1, PASD8, HIF-1-alpha, HIF-1alpha, HIF1-ALPHA, Member of PAS protein 1 |
| Shipping Condition: | RT |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | ESEE122 |
| Isotype: | IgG1 |
| Immunogen: | Human HIF-1 alpha amino acids 329-530. |
| Application: | ICC, IF, IHC-P, IP, WB, IHC-Fr, IHC |
Additional Text
Gene Name
HIF1A
Concentration
1 mg/ml
Gene ID
3091
Antigen Type
Purified protein
Purification
Protein G purified
Short Description
HIF1 Alpha antibody LS-B109 is an unconjugated mouse monoclonal antibody to HIF1 Alpha (HIF1A) (aa329-530) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Storage Note
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Antibody Clonality
Monoclonal
Application Notes
Immunohistochemistry: LS-B109 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B109 was determined to be 1.25 ug/ml.
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