IHC-plus™ Insulin Antibody (clone 2D11.H5)

Catalogue Number: LS-B1445-LSP

Manufacturer:LifeSpan BioSciences Inc.
Preservative:0.01% Sodium azide
Type:Monoclonal Primary Antibody - Unconjugated
Alias:INS, IRDN, IDDM2, Preproinsulin, ILPR, Insulin, MODY10, Proinsulin
Shipping Condition:RT
Unit(s): 50 ug
Host name: Mouse
Clone: 2D11.H5
Isotype: IgG1, kappa
Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with purified human insulin coupled to bovine serum albumin (BSA).
Application: ELISA, IHC-P, IP, WB, DB, IHC

Additional Text

Gene Name

INS

Concentration

0.5 mg/ml

Gene ID

3630

Antigen Type

Fusion protein

Purification

Protein A purified

Short Description

Insulin antibody LS-B1445 is an unconjugated mouse monoclonal antibody to human Insulin. Validated for DB, ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.

Storage Note

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

Antibody Clonality

Monoclonal

Application Notes

Immunohistochemistry: LS-B1445 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1445 was determined to be 5 ug/ml.