IHC-plus™ RAMP3 Antibody
Catalogue Number: LS-B15834-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Shelf Life: | 12 months |
| Preservative: | 0.01% Sodium azide |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | RAMP3 |
| Shipping Condition: | RT |
| Unit(s): | 50 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | |
| Immunogen: | Recombinant full length protein of human RAMP3 |
| Application: | ICC, IF, IHC-P, WB |
Additional Text
Concentration
0.5 mg/ml
Gene Name
RAMP3
Gene ID
10268
Purification
Affinity Purified
Short Description
RAMP3 antibody LS-B15834 is an unconjugated rabbit polyclonal antibody to RAMP3 from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Antibody Clonality
Polyclonal
Storage Note
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Application Notes
Western blot analysis of RAMP3 expression in MCF7 (A), mouse brain (B), mouse heart (C), rat brain (D), rat lung (E) whole cell lysates. Immunofluorescent analysis of RAMP3 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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