IHC-plus™ PCNA Antibody (clone PCNA/694)
Catalogue Number: LS-B16051-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Shelf Life: | 24 months |
| Preservative: | 0.05% Sodium azide |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | PCNA, Cyclin |
| Shipping Condition: | RT |
| Storage Condition: | 2-8°C |
| Unit(s): | 50 ug |
| Host name: | Mouse |
| Clone: | PCNA/694 |
| Isotype: | IgG2a, kappa |
| Immunogen: | Recombinant full length Human protein. |
| Application: | FC, IF, IHC-P, WB |
Additional Text
Gene Name
PCNA
Gene ID
5111
Antigen Type
Recombinant Protein
Purification
Protein G purified
Short Description
PCNA antibody LS-B16051 is an unconjugated mouse monoclonal antibody to human PCNA. Validated for Flow, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Antibody Clonality
Monoclonal
Storage Note
Store at 2°C to 8°C.
Concentration
0.2 mg/ml
Application Notes
Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This mAb is excellent for multiple applications. Optimal dilution of the PCNA antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
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