IHC-plus™ MDM2 Antibody (aa154-167, clone SMP14)
Catalogue Number: LS-B163-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Preservative: | 0.09% Sodium azide |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | MDM2, ACTFS, Double minute 2 protein, Hdm2, MDM2 variant FB30, HDMX, Oncoprotein Mdm2, p53-binding protein Mdm2, MDM2 variant FB28 |
| Shipping Condition: | RT |
| Unit(s): | 50 ug |
| Host name: | Mouse |
| Clone: | SMP14 |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide, CSRPSTSSRRRAISE, corresponding to amino acids 154-167 of human MDM2. Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset, Rat, Dog, Panda (100%); Bat, Cat, Bovine, Hamster, Elephant, Horse, Pig (93%); Mouse (86%). |
| Application: | IHC-P, IHC-Fr, IHC, IHC-R |
Additional Text
Gene Name
MDM2
Concentration
1 mg/ml
Gene ID
4193
Antigen Type
Synthetic Peptide
Purification
Purified
Short Description
MDM2 antibody LS-B163 is an unconjugated mouse monoclonal antibody to human MDM2 (aa154-167). Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Antibody Clonality
Monoclonal
Storage Note
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Application Notes
Immunohistochemistry: LS-B163 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B163 was determined to be 10 ug/ml. Histology Positive Control Tissue: Breast Carcinoma.
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