IHC-plus™ Insulin Antibody (clone 8E2, HRP)
Catalogue Number: LS-B2407-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Preservative: | 0.05% ProClin™ 300 |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Alias: | INS, IRDN, IDDM2, Preproinsulin, ILPR, Insulin, MODY10, Proinsulin |
| Shipping Condition: | RT |
| Unit(s): | 50 ug |
| Host name: | Mouse |
| Clone: | 8E2 |
| Isotype: | IgG1, kappa |
| Immunogen: | Purified human insulin. |
| Application: | ELISA, IHC-P, IHC |
Additional Text
Gene Name
INS
Concentration
0.5 mg/ml
Gene ID
3630
Antigen Type
Purified protein
Purification
Protein A purified
Short Description
Insulin antibody LS-B2407 is an HRP-conjugated mouse monoclonal antibody to human Insulin. Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Antibody Clonality
Monoclonal
Storage Note
Short term: store at 4°C. Long term: aliquot and store -20°C. Avoid freeze-thaw cycles. Protect from light.
Application Notes
Immunohistochemistry: LS-B2407 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2407 was determined to be 10 ug/ml. Recommended as a detection antibody for ELISA and in sandwich assay with clone 3A6 as the capture antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
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