IHC-plus™ HSPG2 / Perlecan Antibody (clone A71)

Catalogue Number: LS-B2468-LSP

Manufacturer:LifeSpan BioSciences Inc.
Preservative:Sodium azide
Type:Monoclonal Primary Antibody - Unconjugated
Alias:HSPG2, Endorepellin (domain V region), HSPG, Heparan sulfate proteoglycan 2, Perlecan, Perlecan proteoglycan, SJA, SJS1, PRCAN, SJS
Shipping Condition:RT
Unit(s): 50 ul
Host name: Mouse
Clone: A71
Isotype: IgG1, kappa
Immunogen: Bovine corneal endothelial cells.
Application: ELISA, IHC-P, IP, WB, IHC-Fr, IHC

Additional Text

Purification

Protein A/G Purified

Antigen Type

Cells

Concentration

1.03 mg/ml

Gene Name

HSPG2

Gene ID

3339

Short Description

Perlecan antibody LS-B2468 is an unconjugated mouse monoclonal antibody to Perlecan (HSPG2) from bovine. It is reactive with human and bovine. Validated for ELISA, IHC, IP and WB.

Storage Note

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

Antibody Clonality

Monoclonal

Application Notes

Immunohistochemistry: LS-B2468 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2468 was determined to be 5 ug/ml.