IHC-plus™ IL1R1 Antibody (Extracellular Domain, clone IL1-31-22.2.1)
Catalogue Number: LS-B2859-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | RT |
| Unit(s): | 50 ug |
| Host name: | Mouse |
| Clone: | IL1-31-22.2.1 |
| Isotype: | IgG2b |
| Immunogen: | The extracellular domain of the type I human IL-1 receptor. |
| Application: | ELISA, IHC-P, RIA, IHC |
Additional Text
Gene ID
3554
Gene Name
IL1R1
Antigen Type
Protein
Purification
Protein G purified
Concentration
1.45 mg/ml
Specificity
IL1R1, Antigen CD121a, D2S1473, IL-1R-1, IL-1R-alpha, IL-1RT-1, IL-1RT1, IL1R, IL1RA, IL1RT1, Interleukin receptor 1, Interleukin-1 receptor alpha, Interleukin-1 receptor type 1, CD121A, CD121a antigen, Interleukin 1 receptor, type I, Interleukin-1 receptor type I, Interleukin-1 type i receptor, p80
Short Description
IL1R1 antibody LS-B2859 is an unconjugated mouse monoclonal antibody to human IL1R1 (Extracellular Domain). Validated for ELISA, IHC and RIA. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Storage Note
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Antibody Clonality
Monoclonal
Application Notes
Immunohistochemistry: LS-B2859 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2859 was determined to be 10 ug/ml. ELISA: Can be used as capture reagents or second antibody at a concentration of 5 ug/ml. RIA: Concentrations of 20-30 ng/ml of these antibodies will bind radiolabeled IL-1 receptor in a radioimmunoassay format. Immunocytochemistry: IL-1 receptor can be detected on cultured cells using antibody concentrations of 10-100 ug/ml.
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