IHC-plus™ ITGB1 / Integrin Beta 1 / CD29 Antibody (clone HUTS-4)

Catalogue Number: LS-B2861-LSP

Manufacturer:LifeSpan BioSciences Inc.
Preservative:No preservative
Type:Monoclonal Primary Antibody - Unconjugated
Alias:ITGB1, CD29, CD29 antigen, Integrin beta-1, MDF2, MSK12, Integrin VLA-4 beta subunit, VLA-BETA, VLAB, Beta 1 integrin, FNRB, GPIIA, Integrin Beta 1, VLA-4 subunit beta
Shipping Condition:RT
Unit(s): 50 ug
Host name: Mouse
Clone: HUTS-4
Isotype: IgG2b
Immunogen: Human b1 integrins purified from Triton X-100 lysates of human lung, liver, and skeletal muscle tissues. Specifically selected due to its increased reactivity with T-lymphoblasts upon Mn2+ stimulation.
Application: ELISA, FC, IHC-P, WB, IHC, IHC-Pfa

Additional Text

Concentration

1 mg/ml

Gene ID

3688

Gene Name

ITGB1

Antigen Type

Cells

Purification

Purified

Short Description

CD29 antibody LS-B2861 is an unconjugated mouse monoclonal antibody to CD29 (ITGB1 / Integrin Beta 1) from human. It is reactive with human and rat. Validated for ELISA, Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.

Storage Note

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

Antibody Clonality

Monoclonal

Application Notes

Immunohistochemistry: LS-B2861 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2861 was determined to be 10 ug/ml. FACS: 2.5 ug/5x10^5 cells.