IHC-plus™ NT5E / eNT / CD73 Antibody (aa27-252, clone 2B6)
Catalogue Number: LS-B2943-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Preservative: | 0.1% sodium azide |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | NT5E, 5-nucleotidase, ecto (CD73), 5'-nucleotidase, 5 nucleotidase (CD73), 5-NT, 5-nucleotidase, 5'-nucleotidase, ecto (CD73), CD73, CD73 antigen, E5NT, Ecto-5-nucleotidase, ENT, EN, NT, Purine 5-Prime-Nucleotidase, NT5, NTE |
| Shipping Condition: | RT |
| Unit(s): | 50 ul |
| Host name: | Mouse |
| Clone: | 2B6 |
| Isotype: | IgG2b, kappa |
| Immunogen: | Recombinant human CD73 (27-252aa) purified from E. coli. |
| Application: | ELISA, IF, IHC-P, SDS-PAGE, WB, IHC |
Additional Text
Concentration
0.5 mg/ml
Gene Name
NT5E
Gene ID
4907
Antigen Type
Recombinant Protein
Purification
Protein G purified
Short Description
CD73 antibody LS-B2943 is an unconjugated mouse monoclonal antibody to human CD73 (NT5E / eNT) (aa27-252). Validated for ELISA, IF, IHC, SDS-PAGE and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Storage Note
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Antibody Clonality
Monoclonal
Application Notes
Immunohistochemistry: LS-B2943 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2943 was determined to be 10 ug/ml.
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