IHC-plus™ F8 / FVIII / Factor VIII Antibody

Catalogue Number: LS-B2979-LSP

Manufacturer:LifeSpan BioSciences Inc.
Type:Polyclonal Primary Antibody - Unconjugated
Alias:F8, AHF, Antihemophilic factor, Coagulation factor VIIIc, Coagulation factor VIII, DXS1253E, Factor VIII F8B, FVIII, HEMA, F8B, Factor VIII, Procoagulant component, F8C
Shipping Condition:RT
Unit(s): 0.5 mg
Host name: Sheep
Clone:
Isotype: IgG
Immunogen: Human Factor VIIIC (F. VIII) purified from F. VIII concentrate.
Application: ELISA, IHC-P, IHC

Additional Text

Concentration

10 mg/mL

Gene Name

F8

Gene ID

2157

Storage Note

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

Antibody Clonality

Polyclonal

Purification

Caprylic Acid Precipitated; Ammonium Sulfate Precipitated; Solid-phase Adsorption

Application Notes

Immunohistochemistry: LS-B2979 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2979 was determined to be 5 ug/ml.

Short Description

Factor VIII antibody LS-B2979 is an unconjugated sheep polyclonal antibody to human Factor VIII (F8 / FVIII). Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.