IHC-plus™ NFATC1 / NFAT2 Antibody (aa428-716, clone AT1C3)
Catalogue Number: LS-B3051-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Preservative: | 0.02% Sodium azide |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | NFATC1, NFATc, NFAT2, NF-ATC, NF-ATC transcription factor, NF-ATc1 |
| Shipping Condition: | RT |
| Unit(s): | 50 ul |
| Host name: | Mouse |
| Clone: | AT1C3 |
| Isotype: | IgG2a, kappa |
| Immunogen: | Recombinant human NFATc1 (428-716aa) purified from E. coli. NCBI Accession No.: NP_765978. |
| Application: | ELISA, IF, IHC-P, SDS-PAGE, WB, IHC |
Additional Text
Gene Name
NFATC1
Concentration
0.5 mg/ml
Gene ID
4772
Antigen Type
Recombinant Protein
Purification
Protein G purified
Short Description
NFAT2 antibody LS-B3051 is an unconjugated mouse monoclonal antibody to human NFAT2 (NFATC1) (aa428-716). Validated for ELISA, IF, IHC, SDS-PAGE and WB. Tested on 20 paraffin-embedded human tissues.
Storage Note
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Antibody Clonality
Monoclonal
Application Notes
Immunohistochemistry: LS-B3051 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3051 was determined to be 10 ug/ml.
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