TFAP2C / AP2 Gamma Antibody (aa252-301)
Catalogue Number: LS-B3506-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Preservative: | 0.09% sodium azide |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | TFAP2C, AP2 Gamma, AP-2 gamma, ERF-1, ERF1, HAP-2g, Transcription factor ERF-1, TFAP2G, AP2-GAMMA, Estrogen receptor factor 1 |
| Shipping Condition: | RT |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide located between aa252-301 of human TFAP2C (Q92754, NP_003213). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Seabass, Salmon, Stickleback, Zebrafish (100%); Panda, Lizard, Drosophila (92%). |
| Application: | IHC-P, WB, IHC |
Additional Text
Gene ID
7022
Gene Name
TFAP2C
Antigen Type
Synthetic Peptide
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Storage Note
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Application Notes
Immunohistochemistry: LS-B3506 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3506 was determined to be 5 ug/ml. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Short Description
AP2 Gamma antibody LS-B3506 is an unconjugated rabbit polyclonal antibody to AP2 Gamma (TFAP2C) (aa252-301) from human. It is reactive with human and mouse. Validated for IHC and WB.
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