IHC-plus™ EIF4EBP1 / 4EBP1 Antibody (aa101-118)

Catalogue Number: LS-B3748-LSP

Manufacturer:	LifeSpan BioSciences Inc.
Type:	Polyclonal Primary Antibody - Unconjugated
Alias:	EIF4EBP1, 4E-BP1, 4EBP1, BP-1, PHAS-I, EIF4E-binding protein 1
Shipping Condition:	RT
Unit(s):	50 ug
Host name:	Rabbit
Clone:
Isotype:
Immunogen:	An 18 residue synthetic peptide SPEDKRAGGEESQFEMDI based on the human PHAS-I (aa 101-118) and the peptide coupled to KLH. This sequence is identical to rat and mouse PHAS- I over these residues. Percent identity by BLAST analysis: Human, Gorilla, Mouse, Rat, Hamster, Rabbit (100%); Marmoset, Goat, Elephant, Dog, Bat, Bovine, Horse (94%); Panda (83%).
Application:	IHC-P, WB, IHC, EMSA

Overview

Additional Text

Gene ID

1978

Gene Name

EIF4EBP1

Concentration

0.9 mg/ml

Purification

Affinity Purified

Antigen Type

Synthetic Peptide, KLH Conjugated

Short Description

4EBP1 antibody LS-B3748 is an unconjugated rabbit polyclonal antibody to 4EBP1 (EIF4EBP1) (aa101-118) from human. It is reactive with human, mouse, rat and other species. Validated for GS, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Storage Note

Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.

Antibody Clonality

Polyclonal

Application Notes

Immunohistochemistry: LS-B3748 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3748 was determined to be 10 ug/ml.

Price

£466.00

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