Catalogue Number: LS-B653-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Preservative: | 0.01% Sodium azide |
| Type: | ChIP Validated Antibodies |
| Alias: | RELA, NF-kappa-B p65delta3, NF-kappaB, NFKB3, p65, Transcription factor p65, NF kappaB, NFKB |
| Shipping Condition: | RT |
| Unit(s): | 50 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | |
| Immunogen: | NFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH). |
| Application: | ELISA, IHC-P, ChIP, WB, IHC, EMSA |
RELA
5970
Synthetic Peptide
80 mg/ml
C-terminus
Delipidated; Defibrinated
NFKB p65 antibody LS-B653 is an unconjugated rabbit polyclonal antibody to NFKB p65 (RELA) (C-Terminus) from human. It is reactive with human, mouse and rat. Validated for ChrIP, ELISA, GS, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 9 publications.
Polyclonal
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Immunohistochemistry: LS-B653 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B653 was determined to be 1:500.