IHC-plus™ NFKB1 / NF-Kappa-B Antibody (N-Terminus)

Catalogue Number: LS-B716-LSP

Manufacturer:LifeSpan BioSciences Inc.
Preservative:0.01% Sodium azide
Type:Polyclonal Primary Antibody - Unconjugated
Alias:NFKB1, Ebp- 1, EBP-1, NF-KappaB p50, NFkappaB, NFKB-p50, KBF1, NF-kappa-B, NF-kappaB, NF-kB1, NFKB-p105, p105, p50, DNA binding factor KBF1, DNA-binding factor KBF1, NF-kappabeta
Shipping Condition:RT
Unit(s): 50 ul
Host name: Rabbit
Clone:
Isotype:
Immunogen: Human NFkB p50 (NFKB1) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Application: ELISA, IHC-P, WB, IHC, EMSA

Additional Text

Gene Name

NFKB1

Gene ID

4790

Antigen Type

Synthetic Peptide

Concentration

85 mg/ml

Protein: Target Region

N-terminus

Purification

Delipidated; Defibrinated

Short Description

NF-Kappa-B antibody LS-B716 is an unconjugated rabbit polyclonal antibody to human NF-Kappa-B (NFKB1) (N-Terminus). Validated for ELISA, GS, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.

Storage Note

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

Antibody Clonality

Polyclonal

Application Notes

Immunohistochemistry: LS-B716 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B716 was determined to be 1:500.