IHC-plus™ B2M / Beta 2 Microglobulin Antibody (clone B2M-01, Concentrated)
Catalogue Number: LS-B8113-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Preservative: | Sodium azide |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | B2M, Beta 2 Microglobulin, Beta-2-microglobulin, Beta-2-microglobin |
| Shipping Condition: | RT |
| Storage Condition: | 2-8°C |
| Unit(s): | 50 ug |
| Host name: | Mouse |
| Clone: | B2M-01 |
| Isotype: | IgG2a |
| Immunogen: | Purified human beta2-microglobulin |
| Application: | ELISA, FC, IHC-P, IP, WB, RIA, IHC |
Additional Text
Concentration
0.5 mg/ml
Gene Name
B2M
Antigen Type
Purified protein
Gene ID
567
Purification
Purified
Short Description
Beta 2 Microglobulin antibody LS-B8113 is an unconjugated mouse monoclonal antibody to human Beta 2 Microglobulin (B2M). Validated for ELISA, Flow, IHC, IP, RIA and WB. Tested on 20 paraffin-embedded human tissues.
Antibody Clonality
Monoclonal
Storage Note
Store at 2-8°C.
Application Notes
For use in immunoprecipitation, this antibody coprecipitates b2-microglobulin (12 kDa) and HLA antigens(45 kDa) from the lysates of human lymphoid cells. Recommend U937 human histiocytic lymphoma cell line orRAJI human Burkitt lymphoma cell line as postitive control and EL4 mouse lymphoblastic lymphoma cell line as negative control for Western blot. Reducing and non-reducing conditions. Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate withnon-reducing/reducing Laemmli SDS-PAGE sample buffer. Recommend peripheral blood lymphocytes as positve control and DAUDI human Burkitt lymphoma cell line, or erythrocytes as negative control for flow cytometry. Recommend kidney glomeruli as postive control for IHC. For RIA, use at an assay dependent concentration. The dissociation constant of the antibody soluble b2-microglobulin is 1.5 x 10-8 mol/l as determined by competitive RIA.
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations