Catalogue Number: LS-C104369-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Physical state: | Lyophilized |
| Type: | Polyclonal Primary Antibody - Conjugated |
| Alias: | LTA, Lymphotoxin-alpha, LT-alpha, TNFSF1, TNF-beta, TNFB, Tumor necrosis factor beta, LT, TNF Beta |
| Shipping Condition: | RT |
| Unit(s): | 25 ug, 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | |
| Immunogen: | E.coli derived recombinant Human TNF-beta. |
| Application: | ELISA, WB, NT |
LTA
4049
Cell extract
Affinity Purified
TNF Beta antibody LS-C104369 is a biotin-conjugated rabbit polyclonal antibody to human TNF Beta (LTA). Validated for ELISA, Neut and WB.
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Polyclonal
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect Human TNF-beta by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human TNF-beta. To detect Human TNF-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human TNF-beta (LS-C104710) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human TNF-beta. Western Blot: To detect Human TNF-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human TNF-beta is 1.5-3 ng/lane, under either reducing or non-reducing conditions.