Catalogue Number: LS-C11037-LSP
Manufacturer: | LifeSpan BioSciences Inc. |
Preservative: | 0.1% Sodium azide |
Type: | Polyclonal Primary Antibody - Unconjugated |
Alias: | VWF, Coagulation factor VIII VWF, F8VWF, VWD, Von Willebrand factor, factor VIII-related antigen, Factor VIII-R, Factor VIII-R Ag, VIII R AG |
Shipping Condition: | RT |
Unit(s): | 1000 ul |
Host name: | Sheep |
Clone: | |
Isotype: | IgG |
Immunogen: | Human von Willebrand Factor Antigen (vWF) prepared from citrated human plasma. |
Application: | ID, IE, IHC-P, IHC |
VWF
Purified protein
7450
3.2 mg/ml
Delipidated
Von Willebrand Factor antibody LS-C11037 is an unconjugated sheep polyclonal antibody to human Von Willebrand Factor (VWF). Validated for ID, IE and IHC.
Polyclonal
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Suitable for use in Immunohistochemistry, Double Diffusion, Counter Current Immunoelectrophoresis (CCIEP) and in a variety of gel techniques. CCIEP: 40 ul antiserum vs. 10 ul plasma, wells 6mm apart. 100v for 30 minutes. The use of 3% PEG 6000 with 1.2% agarose in a suitable buffer (such as TBE or Tris-barbital, pH >8.2) is recommended. Results can be enhanced by washing completed agarose gels in saline, drying and finally staining with a protein stain such as Coomassie blue. Double Diffusion: 20 ul antiserum vs. 5 ul vWF, 1 mg/ml. Immunohistochemistry: Specifically binds to endothelial cells on formalin-fixed, paraffin embedded, sections of human tonsil and pancreas.