Neutrophil Antibody (clone NIMP-R14, Biotin)

Catalogue Number: LS-C121266-LSP

Manufacturer:LifeSpan BioSciences Inc.
Shelf Life:12 months
Preservative:0.02% Sodium azide
Type:Monoclonal Primary Antibody - Conjugated
Shipping Condition:RT
Unit(s): 50 ug
Host name: Rat
Clone: NIMP-R14
Isotype: IgG2b
Immunogen: Murine Ly-6G.
Application: ELISA, FC, IHC-P, IHC-Fr, IHC, FA

Additional Text

Purification

Purified

Short Description

Neutrophil antibody LS-C121266 is a biotin-conjugated rat monoclonal antibody to mouse Neutrophil. Validated for ELISA, Flow, Func and IHC.

Antibody Clonality

Monoclonal

Storage Note

May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for up to 1 year.

Application Notes

Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry and Functional Studies. Flow Cytometry: 1:50 (10 ug/ml) labels 5x10^5 cells. Immunohistochemistry (frozen sections): 1:50 acetone fixation is recommended. Immunohistochemistry (paraffin sections): 1:50 block with 20% normal rabbit serum. Functional Studies (remove sodium azide): Neutrophil depletion. Mice were tested with NIMP-R14 given intraperitoneally at a dose of 1mg, 6 hours before injection. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.