ADAM10 Antibody (CF)

Catalogue Number: LS-C124902-LSP

Manufacturer:LifeSpan BioSciences Inc.
Shelf Life:12 months
Preservative:0.1% sodium azide
Type:Monoclonal Primary Antibody - Conjugated
Alias:ADAM10, AD10, ADAM 10, Kuzbanian, HsT18717, CD156c, CD156c antigen, CDw156, Kuz, Kuzbanian protein homolog, MADM
Shipping Condition:RT
Unit(s): 100 Tests
Host name: Mouse
Clone:
Isotype: IgG2b
Immunogen: Recombinant human ADAM10, amino acids 214-672, UniProt accession #O14672
Application: FC

Additional Text

Gene Name

ADAM10

Gene ID

102

Antigen Type

Recombinant Protein

Purification

Purified

Short Description

ADAM10 antibody LS-C124902 is a CF-conjugated mouse monoclonal antibody to human ADAM10. Validated for Flow.

Antibody Clonality

Monoclonal

Storage Note

May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C. Aliquots are stable for up to 1 year at -20°C.

Application Notes

Suitable for use in Flow cytometry: Intracellular staining antibodies are designed for multiparameter flow cytometric analysis of cells. To stain for surface proteins (e. g. CD3, CD4, CD8) in addition to the intracellular protein, we recommend that the investigator determine whether the fixation and permeabilization steps adversely affect the surface protein. If so, surface staining of cells prior to fixation and permeabilization is recommended. For intracellular staining, cells must first be fixed and permeabilized. Use of 4% paraformaldehyde in PBS as a fixative is recommended. Other formulations or tissue fixatives may affect the staining properties of the monoclonal antibody. For permeabilization, 0.1% saponin in a balanced salt solution is effective in facilitating antibody entry into cells. Due to the reversible nature of cell membrane permeabilization, saponin must be included in all buffers used (i. e. both the staining and washing steps). The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.