CD3 + CD8 + CD45 Antibody (clone BHPT-1, FITC, PE, Cy5)
Catalogue Number: LS-C140483-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Preservative: | 0.08% Sodium azide |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Shipping Condition: | RT |
| Unit(s): | 50 Tests |
| Host name: | Mouse |
| Clone: | BHPT-1 |
| Isotype: | |
| Immunogen: | |
| Application: | FC |
Additional Text
Antigen Type
Recombinant Protein
Purification
Affinity Purified
Short Description
CD3 + CD8 + CD45 antibody LS-C140483 is an FITC, PE, Cy5-conjugated mouse monoclonal antibody to human CD3 + CD8 + CD45. Validated for Flow.
Storage Note
Store at 4°C. Do not freeze.
Antibody Clonality
Monoclonal
Application Notes
PBMC: Add 10 ul of 3-ColorTM antibody reagent/10^6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2 to 8°C. Wash twice with PBS. Add 10 ul of Exalpha Biological's pre-titered 3-ColorTM and incubate 15 minutes at 2 to 8°C. Wash twice with PBS or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 ul of Exalpha Biological's 3-ColorTM antibody reagent MAB/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature 20°C. Lyse the whole blood. Wash once with PBS and add 10 ul of Exalpha Biological's 3-ColorTM and incubate 15 minutes at 2 to 8°C. Wash twice with PBS or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
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