AKT1 Antibody (phospho-Ser473, clone 17F6.B11, Atto 594)
Catalogue Number: LS-C153792-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Preservative: | 0.01% Sodium azide |
| Physical state: | Lyophilized |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Alias: | AKT1, AKT, AKT-1, C-AKT, Pan-AKT, PKB alpha, PKBalpha, Proto-oncogene c-Akt, Protein kinase B, Rac protein kinase alpha, PRKBA, Protein kinase B alpha, RAC-PK-alpha, PKB, PKB-ALPHA, RAC, RAC-ALPHA |
| Shipping Condition: | RT |
| Unit(s): | 100 ug |
| Host name: | Mouse |
| Clone: | 17F6.B11 |
| Isotype: | IgG1, kappa |
| Immunogen: | Akt phospho S473 ATTO594 Conjugated Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein. |
| Application: | ELISA, FC, IF, WB, FLISA |
Additional Text
Gene Name
AKT1
Concentration
0.5 mg/ml
Gene ID
207
Antigen Type
Synthetic Peptide
Modification
Phosphorylated, Ser473
Purification
Protein A purified
Short Description
AKT1 antibody LS-C153792 is an Atto 594-conjugated mouse monoclonal antibody to AKT1 (pSer473) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, FLISA, Flow, IF and WB.
Storage Note
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Antibody Clonality
Monoclonal
Application Notes
Anti-AKT pS473 Antibody ATTO 594 Conjugated is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (ELISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Expect a band approximately 56 kD in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
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