IL-1B / IL-1 Beta Antibody (N-Terminus, Biotin)
Catalogue Number: LS-C154117-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Preservative: | 0.01% Sodium azide |
| Physical state: | Lyophilized |
| Type: | Polyclonal Primary Antibody - Conjugated |
| Alias: | IL1B, IL-1B, IL-1 beta, IL1-BETA, Preinterleukin 1 beta, Pro-interleukin-1-beta, Catabolin, IL-1, IL1F2, Interleukin 1, beta, Interleukin-1 beta |
| Shipping Condition: | RT |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | This antibody was prepared by repeated immunizations with recombinant human IL-1β produced in E.coli. The MW of the recombinant 153 aa IL-1β was 17 kDa with the N-terminal amino acid at position alanine 117. This cleavage site is generated by the IL-1β converting enzyme (ICE, capase-1). |
| Application: | ELISA, FC, IF, IP, WB, RIA, IHC, NT |
Additional Text
Gene Name
IL1B
Concentration
0.5 mg/ml
Gene ID
3553
Antigen Type
Recombinant Protein
Protein: Target Region
N-terminus
Purification
Ion Exchange
Short Description
IL-1 Beta antibody LS-C154117 is a biotin-conjugated rabbit polyclonal antibody to IL-1 Beta (IL-1B) (N-Terminus) from human. It is reactive with human, dog and primate. Validated for ELISA, Flow, IF, IHC, IP, Neut, RIA and WB.
Storage Note
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Antibody Clonality
Polyclonal
Application Notes
Anti-Human IL-1beta has been tested for use in neutralizations, ELISA, radioimmunoassays, flow cytometry, immunohistochemistry, immunoblotting and immunoprecipitation. It recognizes the 17000 MW mature IL-1beta. For immunoblots, typically, IL-1beta is detected from supernatants or lysates of 2 x 10E6 endotoxin-stimulated peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1% (v/v) serum plus 10 ng/mL E. coli LPS. For immunoprecipitation pre-clearing the preparation with a non-specific Rabbit IgG (p/n 011-001-297) to reduce background is suggested. For immunohistochemistry either paraffin fixation or cryofixation can be used for sample preparation to stain intracellular IL-1beta. For ELISA use HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (611-1302) for detection. In ELISA formats this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. This antibody is also useful for neutralization of human and primate IL-1beta activity in bioassays. It does not neutralize the biological activity IL-1a. It does not neutralize the biological activity of murine, rat or rabbit IL-1beta. For neutralization, it is recommended to incubate the sample with a dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. This antibody can be used for FACS analysis. Caution should be exhibited as the F( c) domain of the rabbit IgG molecule may interact with cells non-specifically. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
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