GFP Antibody (AP)
Catalogue Number: LS-C154196-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Preservative: | 0.1% sodium azide |
| Type: | Polyclonal Primary Antibody - Conjugated |
| Alias: | GFP |
| Shipping Condition: | RT |
| Unit(s): | 1 mg |
| Host name: | Goat |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria. |
| Application: | ELISA, IF, WB, IHC |
Additional Text
Concentration
0.5 mg/ml
Antigen Type
Fusion protein
Purification
Affinity Purified
Short Description
GFP antibody LS-C154196 is an AP-conjugated goat polyclonal antibody to aequorea victoria GFP. Validated for ELISA, IF, IHC and WB.
Storage Note
Store at 4°C. Do not freeze.
Antibody Clonality
Polyclonal
Application Notes
Anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with a monoclonal anti-GFP antibody using either form of the antibody as the capture or detection antibodies. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E. coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
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