GFP Antibody (clone 9F9.F9, TR)

Catalogue Number: LS-C154213-LSP

Manufacturer:	LifeSpan BioSciences Inc.
Preservative:	0.01% Sodium azide
Physical state:	Lyophilized
Type:	Monoclonal Primary Antibody - Conjugated
Alias:	GFP
Shipping Condition:	RT
Unit(s):	1 mg
Host name:	Mouse
Clone:	9F9.F9
Isotype:	IgG1, kappa
Immunogen:	Anti-Green Fluorescent Protein (GFP) is produced by immunizing GFP containing fusion protein that corresponds to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Application:	IF, WB, DB, FLISA

Overview

Additional Text

Concentration

0.5 mg/ml

Antigen Type

Fusion protein

Purification

Protein A purified

Short Description

GFP antibody LS-C154213 is a TR-conjugated mouse monoclonal antibody to aequorea victoria GFP. Validated for DB, FLISA, IF and WB.

Storage Note

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

Antibody Clonality

Monoclonal

Application Notes

Monoclonal anti-GFP is designed to detect enhanced GFP and GFP containing recombinant proteins. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated monoclonal anti-GFP is well suited to titrate GFP in a sandwich ELISA in combination with a polyclonal anti-GFP antibody as the capture antibody. Only use the monoclonal form for the detection of enhanced or recombinant GFP. Polyclonal anti-GFP detects all variants of GFP tested to date. The biotin conjugated detection antibody is typically used with streptavidin conjugated HRP or other streptavidin conjugates. The use of polyclonal anti-GFP results in significant amplification of signal when fluorochrome conjugated polyclonal anti-GFP is used relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Price

£1,178.00

-

Quantity
Unit Size

Find out about delivery