RFX5 Antibody

Catalogue Number: LS-C18752-LSP

Manufacturer:LifeSpan BioSciences Inc.
Preservative:0.01% Sodium azide
Type:ChIP Validated Antibodies
Alias:RFX5, Binding regulatory factor, DNA-binding protein RFX5, Regulatory factor X 5
Shipping Condition:RT
Unit(s): 100 ug
Host name: Rabbit
Clone:
Isotype: IgG
Immunogen: RFX5 peptide corresponding to a region at amino acids 320 to 494 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Application: ELISA, ChIP, WB, EMSA

Additional Text

Concentration

0.5 mg/ml

Gene Name

RFX5

Antigen Type

Native protein

Gene ID

5993

Short Description

RFX5 antibody LS-C18752 is an unconjugated rabbit polyclonal antibody to human RFX5. Validated for ChrIP, ELISA, GS and WB.

Antibody Clonality

Polyclonal

Storage Note

Store vial at -20°C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

Purification

Delipidated; Salt Fractionated; Ion Exchange

Application Notes

Suitable for immunoprecipitation, immunoblotting, ELISA and supershift assays. This product was assayed by immunoblot and found to be reactive against RFX5 (aa 320 to 494) from a variety of fibroblast and B-cell lysates at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) catalog no. LS-C60884. Anti- RFX5 (aa 320 to 494) detects a 75 kD band by immunoblot for human RFX5. This product was also tested in a gel supershift assay and found to be reactive against RFX5 complexes using 0.5 to 1.0 ul per assay.