Catalogue Number: LS-C18752-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Preservative: | 0.01% Sodium azide |
| Type: | ChIP Validated Antibodies |
| Alias: | RFX5, Binding regulatory factor, DNA-binding protein RFX5, Regulatory factor X 5 |
| Shipping Condition: | RT |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | RFX5 peptide corresponding to a region at amino acids 320 to 494 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH). |
| Application: | ELISA, ChIP, WB, EMSA |
0.5 mg/ml
RFX5
Native protein
5993
RFX5 antibody LS-C18752 is an unconjugated rabbit polyclonal antibody to human RFX5. Validated for ChrIP, ELISA, GS and WB.
Polyclonal
Store vial at -20°C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Delipidated; Salt Fractionated; Ion Exchange
Suitable for immunoprecipitation, immunoblotting, ELISA and supershift assays. This product was assayed by immunoblot and found to be reactive against RFX5 (aa 320 to 494) from a variety of fibroblast and B-cell lysates at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) catalog no. LS-C60884. Anti- RFX5 (aa 320 to 494) detects a 75 kD band by immunoblot for human RFX5. This product was also tested in a gel supershift assay and found to be reactive against RFX5 complexes using 0.5 to 1.0 ul per assay.