IL-1B / IL-1 Beta Antibody (N-Terminus)
Catalogue Number: LS-C18827-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | IL1B, IL-1B, IL-1 beta, IL1-BETA, Preinterleukin 1 beta, Pro-interleukin-1-beta, Catabolin, IL-1, IL1F2, Interleukin 1, beta, Interleukin-1 beta |
| Shipping Condition: | RT |
| Unit(s): | 1 mg |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | This antibody was prepared by repeated immunizations with recombinant human IL-1β produced in E.coli. The MW of the recombinant 153 aa IL-1β was 17 kDa with the N-terminal amino acid at position alanine 117. This cleavage site is generated by the IL-1β converting enzyme (ICE, capase-1). |
| Application: | ELISA, FC, IF, IHC-P, IP, WB, RIA, IHC-Fr, IHC, NT |
Additional Text
Gene Name
IL1B
Gene ID
3553
Concentration
2 mg/ml
Antigen Type
Recombinant Protein
Protein: Target Region
N-terminus
Purification
Ion Exchange
Short Description
IL-1 Beta antibody LS-C18827 is an unconjugated rabbit polyclonal antibody to IL-1 Beta (IL-1B) (N-Terminus) from human. It is reactive with human, dog and primate. Validated for ELISA, Flow, IF, IHC, IP, Neut, RIA and WB. Cited in 2 publications.
Antibody Clonality
Polyclonal
Storage Note
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Application Notes
This IgG fraction antibody of anti- Human IL-1beta has been tested for use in neutralizations, ELISA, radioimmunoassays, immunohistochemistry, immunoblotting and immunoprecipitation. It recognizes the 17000 MW mature IL-10. For immunoblots, typically, IL-1beta is detected from supernatants or lysates of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1 % (v/v) human serum plus 10 ng/mL E. coli LPS. For immunoprecipitation a dilution of 1:400 to 1:800 is suggested. Pre-clearing the preparation with a non-specific rabbit IgG (011-001-297) to reduce background. For immunohistochemistry a dilution of 1:100 to 1:200 is suggested. Either paraffin fixation or cryofixation can be used for sample preparation to stain intracellular IL- 1beta. For ELISA also use HRP Conjugated Anti-Rabbit IgG [HalphaL] (Goat) (611-1302) for detection.
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