IL1RN Antibody
Catalogue Number: LS-C18829-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Preservative: | 0.01% Sodium azide |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | IL1RN, DIRA, IL-1ra, IL-1RN, IL1F3, IL1RA, MVCD4, IL1 inhibitor, IL1RN (IL1F3), IRAP, ICIL-1RA, IL-1ra3 |
| Shipping Condition: | RT |
| Unit(s): | 1 mg |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human sIL-1Ra produced in E.coli. |
| Application: | ELISA, FC, WB, RIA, IHC, NT |
Additional Text
Concentration
0.5 mg/ml
Gene ID
3557
Gene Name
IL1RN
Antigen Type
Recombinant Protein
Purification
Ion Exchange
Short Description
IL1RN antibody LS-C18829 is an unconjugated rabbit polyclonal antibody to human IL1RN. Validated for ELISA, Flow, IHC, Neut, RIA and WB.
Antibody Clonality
Polyclonal
Storage Note
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Application Notes
This IgG fraction antibody of anti-Human IL-1 Receptor Antagonist has been tested for use in neutralizations, ELISA, radioimmunoassays, western blotting, immunohistochemistry and flow cytometry. Reactivity in other immunoassays is unknown. This product has been assayed by immunoblot using HRP Goat-anti-Rabbit IgG [H&L] (catalog no. LS-C60884) and TMB as a substrate. A working dilution range of 1:400 to 1:800 is suggested for this application. This product has been assayed by ELISA against IL-1 Receptor Antagonist using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (catalog no. LS-C60884) and ABTS as a substrate for 30 minutes at room temperature. A working dilution range of 1:500 to 1:1000 is suggested for this product. For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. This product has been assayed by immunohistochemistry. A dilution of 1:400 or greater is suggested for this immunoassay. Either paraffin fixation or cryofixation can be used for immunohistochemistry. This product has been assayed by radioimmunoassay against antigen. A dilution of 1:8000 is suggested for this immunoassay.
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