PDYN / ProDynorphin Antibody (aa232-245)
Catalogue Number: LS-C22974-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Preservative: | 0.05% Sodium azide |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | PDYN, ADCA, Beta-neoendorphin-dynorphin, Leumorphin, Preprodynorphin, Spinocerebellar ataxia 23, PENKB, Prodynorphin, Rimorphin, SCA23, Leu-enkephalin, Preproenkephalin B, Proenkephalin-B |
| Shipping Condition: | RT |
| Unit(s): | 50 ul |
| Host name: | Guinea Pig |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to residues 232-245 of guinea pig proDynorphin. |
| Application: | IF, IHC |
Additional Text
Gene Name
PDYN
Antigen Type
Synthetic Peptide
Gene ID
5173
Short Description
ProDynorphin antibody LS-C22974 is an unconjugated guinea pig polyclonal antibody to guinea pig ProDynorphin (PDYN) (aa232-245). Validated for IF and IHC.
Antibody Clonality
Polyclonal
Storage Note
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Application Notes
Suitable for use in Immunohistochemistry. Immunohistochemistry: 1:500. Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode's solution, 2) fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4?C with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy.
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